Liquid Chromatography with Mass Spectroscopy:
Incubation:Article Title: Protein phosphatase 2A controls ongoing DNA replication by binding to and regulating cell division cycle 45 (CDC45)
Article Snippet: On the day of flow cytometry analysis, DNA is harvest by centrifugation at 1200 xg's for 7 minutes at 4° C. Samples are washed twice in cold PBS and resuspended in 400 μL of PBS with 50 μg/mL propidium iodide (Signma Aldrich, MA, USA) and 100 μg/mL RNase A (Signma Aldrich, MA, USA). .. Cell are incubated in dark for 20 minutes, before transfering into filter tip cytometry tubes (Fisher Scientific, MA, USA). ..
Cytometry:Article Title: Protein phosphatase 2A controls ongoing DNA replication by binding to and regulating cell division cycle 45 (CDC45)
Article Snippet: On the day of flow cytometry analysis, DNA is harvest by centrifugation at 1200 xg's for 7 minutes at 4° C. Samples are washed twice in cold PBS and resuspended in 400 μL of PBS with 50 μg/mL propidium iodide (Signma Aldrich, MA, USA) and 100 μg/mL RNase A (Signma Aldrich, MA, USA). .. Cell are incubated in dark for 20 minutes, before transfering into filter tip cytometry tubes (Fisher Scientific, MA, USA). ..
other:
Dissection:Article Title: A Novel Method for Detecting Duchenne Muscular Dystrophy in Blood Serum of mdx Mice.
Article Snippet: • The mdx mouse model of Duchenne muscular dystrophy (C57BL/10ScSn-Dmd/J; The Jackson laboratory, Bar Harbor, ME, USA St ck number 001801) and the counterpart wild type control mouse (C57BL/10ScSnJ; The Jackson laboratory, St ck number 000476). .. • 70% Isopropyl alcohol (Fisher Scientific, Waltham, MA, USA, cat. No. A459-500) • Cotton swabs (Texwipe, Kernersville, NC, USA, cat. no. 18-366-473) • Dissection tray (EiscoLabs, Victor, NY, USA, cat. no. 2021884) • Sharp dissecting scissors (VWR, Radnor, PA, USA, cat. no 82027-578-EA) • Forceps (Fisher Scientific, cat. No. 08-895) • A 25 gauge needle (BD Syringe, East Rutherford, NJ, USA, cat no. 801015) • Gilson pipettes (Gilson, Middleton, WI, USA, cat. no. F123601) • Autoclaved tip ( ilson, cat. no F172310) • 0.5 mL Eppendorf tubes (Axygen, Union City, CA, USA, cat. no. PCR05B) • Plain glass microscope slides (Fisher Scientific, cat. no. 12-550-A3) • Aluminum foil tape (ULINE, Pleasant Prairie, WI, USA, cat. no. S-18880) • Acetone (Thermo Fisher Scientific, cat. no. A18-500) • Kimtech scienceTM KimwipesTM delicate task wipes (Kimberly-Clark Professional, Roswell, GA, USA, product code 34155) • Petri dish with clear lid (Fisher Scientific, cat. no. FB0875713) • EppendorfTM 0.5–10 μL single-channel pipette (Fisher Scientific, cat. no. 05-412-422) • Filter tip pipette tips (Fisher Scientific, cat. no. 02-707-473) .. • EZ Anesthesia AF 9000 Auto flow Anesthesia System (EZ Systems, Palmer, PA, USA, cat. no. EZ-190AF) • Universal Animal Restrainer (VWR, cat. no. 47750-270) • Induction chamber for isoflurane inhalation (Vet Equip, Pleasanton, CA, USA, cat. no. 942102) • Refrigerated tabletop centrifuge (Fisher Scientific, cat. no. 22-029-677) • Ultra-low temperature freezer (Fisher Scientific, cat. no TSX50086A) • Renishaw inViaTM confocal Raman microscope (Renishaw, Wotton-under-Edge, England, UK) • MATLAB® software (MathWorks, Natick, MA, USA) • PLS_Toolbox software (Eigenvector Research Inc, Ma son, WA, USA) • easyROC web-tool ver.
Article Title: A Novel Method for Detecting Duchenne Muscular Dystrophy in Blood Serum of mdx Mice.
Article Snippet: Materials and Reagents • The mdx mouse model of Duchenne muscular dystrophy (C57BL/10ScSn- Dmd/J; The Jackson laboratory, Bar Harbor, ME, USA Stock number 001801) and the counterpart wild type control mouse (C57BL/10ScSnJ; The Jackson laboratory, Stock number 000476). .. • 70% Isopropyl alcohol (Fisher Scientific, Waltham, MA, USA, cat. No. A459-500) • Cotton swabs (Texwipe, Kernersville, NC, USA, cat. no. 18-366-473) • Dissection tray (EiscoLabs, Victor, NY, USA, cat. no. 2021884) • Sharp dissecting scissors (VWR, Radnor, PA, USA, cat. no 82027-578-EA) • Forceps (Fisher Scientific, cat. No. 08-895) • A 25 gauge needle (BD Syringe, East Rutherford, NJ, USA, cat no. 801015) • Gilson pipettes (Gilson, Middleton, WI, USA, cat. no. F123601) • Autoclaved tips (Gilson, cat. no F172310) • 0.5 mL Eppendorf tubes (Axygen, Union City, CA, USA, cat. no. PCR05B) • Plain glass microscope slides (Fisher Scientific, cat. no. 12-550-A3) • Aluminum foil tape (ULINE, Pleasant Prairie, WI, USA, cat. no. S-18880) • Acetone (Thermo Fisher Scientific, cat. no. A18-500) • Kimtech scienceTM KimwipesTM delicate task wipes (Kimberly-Clark Professional, Roswell, GA, USA, product code 34155) • Petri dish with clear lid (Fisher Scientific, cat. no. FB0875713) • EppendorfTM 0.5–10 μL single-channel pipette (Fisher Scientific, cat. no. 05-412-422) • Filter tip pipette tips (Fisher Scientific, cat. no. 02-707-473) 2.2. .. Equipment and Software • EZ Anesthesia AF 9000 Auto flow Anesthesia System (EZ Systems, Palmer, PA, USA, cat. no. EZ-190AF) • Universal Animal Restrainer (VWR, cat. no. 47750-270) • Induction chamber for isoflurane inhalation (Vet Equip, Pleasanton, CA, USA, cat. no. 942102) • Refrigerated tabletop centrifuge (Fisher Scientific, cat. no. 22-029-677) • Ultra-low temperature freezer (Fisher Scientific, cat. no TSX50086A) • Renishaw inViaTM confocal Raman microscope (Renishaw, Wotton-under-Edge, England, UK) • MATLAB® software (MathWorks, Natick, MA, USA) • PLS_Toolbox software (Eigenvector Research Inc, Manson, WA, USA) Figure 1.
Microscopy:Article Title: A Novel Method for Detecting Duchenne Muscular Dystrophy in Blood Serum of mdx Mice.
Article Snippet: • The mdx mouse model of Duchenne muscular dystrophy (C57BL/10ScSn-Dmd/J; The Jackson laboratory, Bar Harbor, ME, USA St ck number 001801) and the counterpart wild type control mouse (C57BL/10ScSnJ; The Jackson laboratory, St ck number 000476). .. • 70% Isopropyl alcohol (Fisher Scientific, Waltham, MA, USA, cat. No. A459-500) • Cotton swabs (Texwipe, Kernersville, NC, USA, cat. no. 18-366-473) • Dissection tray (EiscoLabs, Victor, NY, USA, cat. no. 2021884) • Sharp dissecting scissors (VWR, Radnor, PA, USA, cat. no 82027-578-EA) • Forceps (Fisher Scientific, cat. No. 08-895) • A 25 gauge needle (BD Syringe, East Rutherford, NJ, USA, cat no. 801015) • Gilson pipettes (Gilson, Middleton, WI, USA, cat. no. F123601) • Autoclaved tip ( ilson, cat. no F172310) • 0.5 mL Eppendorf tubes (Axygen, Union City, CA, USA, cat. no. PCR05B) • Plain glass microscope slides (Fisher Scientific, cat. no. 12-550-A3) • Aluminum foil tape (ULINE, Pleasant Prairie, WI, USA, cat. no. S-18880) • Acetone (Thermo Fisher Scientific, cat. no. A18-500) • Kimtech scienceTM KimwipesTM delicate task wipes (Kimberly-Clark Professional, Roswell, GA, USA, product code 34155) • Petri dish with clear lid (Fisher Scientific, cat. no. FB0875713) • EppendorfTM 0.5–10 μL single-channel pipette (Fisher Scientific, cat. no. 05-412-422) • Filter tip pipette tips (Fisher Scientific, cat. no. 02-707-473) .. • EZ Anesthesia AF 9000 Auto flow Anesthesia System (EZ Systems, Palmer, PA, USA, cat. no. EZ-190AF) • Universal Animal Restrainer (VWR, cat. no. 47750-270) • Induction chamber for isoflurane inhalation (Vet Equip, Pleasanton, CA, USA, cat. no. 942102) • Refrigerated tabletop centrifuge (Fisher Scientific, cat. no. 22-029-677) • Ultra-low temperature freezer (Fisher Scientific, cat. no TSX50086A) • Renishaw inViaTM confocal Raman microscope (Renishaw, Wotton-under-Edge, England, UK) • MATLAB® software (MathWorks, Natick, MA, USA) • PLS_Toolbox software (Eigenvector Research Inc, Ma son, WA, USA) • easyROC web-tool ver.
Article Title: A Novel Method for Detecting Duchenne Muscular Dystrophy in Blood Serum of mdx Mice.
Article Snippet: Materials and Reagents • The mdx mouse model of Duchenne muscular dystrophy (C57BL/10ScSn- Dmd/J; The Jackson laboratory, Bar Harbor, ME, USA Stock number 001801) and the counterpart wild type control mouse (C57BL/10ScSnJ; The Jackson laboratory, Stock number 000476). .. • 70% Isopropyl alcohol (Fisher Scientific, Waltham, MA, USA, cat. No. A459-500) • Cotton swabs (Texwipe, Kernersville, NC, USA, cat. no. 18-366-473) • Dissection tray (EiscoLabs, Victor, NY, USA, cat. no. 2021884) • Sharp dissecting scissors (VWR, Radnor, PA, USA, cat. no 82027-578-EA) • Forceps (Fisher Scientific, cat. No. 08-895) • A 25 gauge needle (BD Syringe, East Rutherford, NJ, USA, cat no. 801015) • Gilson pipettes (Gilson, Middleton, WI, USA, cat. no. F123601) • Autoclaved tips (Gilson, cat. no F172310) • 0.5 mL Eppendorf tubes (Axygen, Union City, CA, USA, cat. no. PCR05B) • Plain glass microscope slides (Fisher Scientific, cat. no. 12-550-A3) • Aluminum foil tape (ULINE, Pleasant Prairie, WI, USA, cat. no. S-18880) • Acetone (Thermo Fisher Scientific, cat. no. A18-500) • Kimtech scienceTM KimwipesTM delicate task wipes (Kimberly-Clark Professional, Roswell, GA, USA, product code 34155) • Petri dish with clear lid (Fisher Scientific, cat. no. FB0875713) • EppendorfTM 0.5–10 μL single-channel pipette (Fisher Scientific, cat. no. 05-412-422) • Filter tip pipette tips (Fisher Scientific, cat. no. 02-707-473) 2.2. .. Equipment and Software • EZ Anesthesia AF 9000 Auto flow Anesthesia System (EZ Systems, Palmer, PA, USA, cat. no. EZ-190AF) • Universal Animal Restrainer (VWR, cat. no. 47750-270) • Induction chamber for isoflurane inhalation (Vet Equip, Pleasanton, CA, USA, cat. no. 942102) • Refrigerated tabletop centrifuge (Fisher Scientific, cat. no. 22-029-677) • Ultra-low temperature freezer (Fisher Scientific, cat. no TSX50086A) • Renishaw inViaTM confocal Raman microscope (Renishaw, Wotton-under-Edge, England, UK) • MATLAB® software (MathWorks, Natick, MA, USA) • PLS_Toolbox software (Eigenvector Research Inc, Manson, WA, USA) Figure 1.
Transferring:Article Title: A Novel Method for Detecting Duchenne Muscular Dystrophy in Blood Serum of mdx Mice.
Article Snippet: • The mdx mouse model of Duchenne muscular dystrophy (C57BL/10ScSn-Dmd/J; The Jackson laboratory, Bar Harbor, ME, USA St ck number 001801) and the counterpart wild type control mouse (C57BL/10ScSnJ; The Jackson laboratory, St ck number 000476). .. • 70% Isopropyl alcohol (Fisher Scientific, Waltham, MA, USA, cat. No. A459-500) • Cotton swabs (Texwipe, Kernersville, NC, USA, cat. no. 18-366-473) • Dissection tray (EiscoLabs, Victor, NY, USA, cat. no. 2021884) • Sharp dissecting scissors (VWR, Radnor, PA, USA, cat. no 82027-578-EA) • Forceps (Fisher Scientific, cat. No. 08-895) • A 25 gauge needle (BD Syringe, East Rutherford, NJ, USA, cat no. 801015) • Gilson pipettes (Gilson, Middleton, WI, USA, cat. no. F123601) • Autoclaved tip ( ilson, cat. no F172310) • 0.5 mL Eppendorf tubes (Axygen, Union City, CA, USA, cat. no. PCR05B) • Plain glass microscope slides (Fisher Scientific, cat. no. 12-550-A3) • Aluminum foil tape (ULINE, Pleasant Prairie, WI, USA, cat. no. S-18880) • Acetone (Thermo Fisher Scientific, cat. no. A18-500) • Kimtech scienceTM KimwipesTM delicate task wipes (Kimberly-Clark Professional, Roswell, GA, USA, product code 34155) • Petri dish with clear lid (Fisher Scientific, cat. no. FB0875713) • EppendorfTM 0.5–10 μL single-channel pipette (Fisher Scientific, cat. no. 05-412-422) • Filter tip pipette tips (Fisher Scientific, cat. no. 02-707-473) .. • EZ Anesthesia AF 9000 Auto flow Anesthesia System (EZ Systems, Palmer, PA, USA, cat. no. EZ-190AF) • Universal Animal Restrainer (VWR, cat. no. 47750-270) • Induction chamber for isoflurane inhalation (Vet Equip, Pleasanton, CA, USA, cat. no. 942102) • Refrigerated tabletop centrifuge (Fisher Scientific, cat. no. 22-029-677) • Ultra-low temperature freezer (Fisher Scientific, cat. no TSX50086A) • Renishaw inViaTM confocal Raman microscope (Renishaw, Wotton-under-Edge, England, UK) • MATLAB® software (MathWorks, Natick, MA, USA) • PLS_Toolbox software (Eigenvector Research Inc, Ma son, WA, USA) • easyROC web-tool ver.
Article Title: A Novel Method for Detecting Duchenne Muscular Dystrophy in Blood Serum of mdx Mice.
Article Snippet: Materials and Reagents • The mdx mouse model of Duchenne muscular dystrophy (C57BL/10ScSn- Dmd/J; The Jackson laboratory, Bar Harbor, ME, USA Stock number 001801) and the counterpart wild type control mouse (C57BL/10ScSnJ; The Jackson laboratory, Stock number 000476). .. • 70% Isopropyl alcohol (Fisher Scientific, Waltham, MA, USA, cat. No. A459-500) • Cotton swabs (Texwipe, Kernersville, NC, USA, cat. no. 18-366-473) • Dissection tray (EiscoLabs, Victor, NY, USA, cat. no. 2021884) • Sharp dissecting scissors (VWR, Radnor, PA, USA, cat. no 82027-578-EA) • Forceps (Fisher Scientific, cat. No. 08-895) • A 25 gauge needle (BD Syringe, East Rutherford, NJ, USA, cat no. 801015) • Gilson pipettes (Gilson, Middleton, WI, USA, cat. no. F123601) • Autoclaved tips (Gilson, cat. no F172310) • 0.5 mL Eppendorf tubes (Axygen, Union City, CA, USA, cat. no. PCR05B) • Plain glass microscope slides (Fisher Scientific, cat. no. 12-550-A3) • Aluminum foil tape (ULINE, Pleasant Prairie, WI, USA, cat. no. S-18880) • Acetone (Thermo Fisher Scientific, cat. no. A18-500) • Kimtech scienceTM KimwipesTM delicate task wipes (Kimberly-Clark Professional, Roswell, GA, USA, product code 34155) • Petri dish with clear lid (Fisher Scientific, cat. no. FB0875713) • EppendorfTM 0.5–10 μL single-channel pipette (Fisher Scientific, cat. no. 05-412-422) • Filter tip pipette tips (Fisher Scientific, cat. no. 02-707-473) 2.2. .. Equipment and Software • EZ Anesthesia AF 9000 Auto flow Anesthesia System (EZ Systems, Palmer, PA, USA, cat. no. EZ-190AF) • Universal Animal Restrainer (VWR, cat. no. 47750-270) • Induction chamber for isoflurane inhalation (Vet Equip, Pleasanton, CA, USA, cat. no. 942102) • Refrigerated tabletop centrifuge (Fisher Scientific, cat. no. 22-029-677) • Ultra-low temperature freezer (Fisher Scientific, cat. no TSX50086A) • Renishaw inViaTM confocal Raman microscope (Renishaw, Wotton-under-Edge, England, UK) • MATLAB® software (MathWorks, Natick, MA, USA) • PLS_Toolbox software (Eigenvector Research Inc, Manson, WA, USA) Figure 1.
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